ChIP-Grade Protein G Agarose Beads from Cell Signaling Technology

Product Specs
ItemChIP-Grade Protein G Agarose Beads
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9007S
Quantity1 ml
ApplicationsChIP
Research AreaRelated Products
TypeResins
ModeAntibody Purification

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Echinococcus granulosus cyst fluid inhibits inflammatory responses through inducing histone demethylase KDM5B in macrophagesParasites & VectorsSeptember 9, 2023Xiaopeng Wang, Ruolin Lin, Chunxue Fu, Chun Yang, Dan Dong, Xiangwei Wu, Xueling Chen, Lianghai Wang, Jun Houprepared by incubating with specific primary Ab and anti-IgG overnight at 4 °C and precipitated using ChIP-Grade Protein G Agarose Beads. Following the reversal of the protein-DNA crosslinking, DNA was purified using DNA Purification Buffers

(2) Diabetes impairs fracture healing through Foxo1 mediated disruption of ciliogenesisCell Death DiscoveryAugust 17, 2023Zahra Chinipardaz, Gongsheng Yuan, Min Liu, Dana T. Graves, Shuying Yang(Cell Signaling, 2729). Protein-DNA complexes were purified using protein G Agarose beads (Cell Signaling, 9007). RT-qPCR was performed with IFT80 promoter primers [IFT80F (5′-TGGTCGCAGGACAGCCTTTG-3′) and IFT80R

(3) The Histone Deacetylases Hst1 and Rpd3 Integrate De Novo NAD+ Metabolism with Phosphate Sensing in Saccharomyces cerevisiaeInternational Journal of Molecular SciencesApril 28, 2023Benjamin Groth, Yi-Ching Lee, Chi-Chun Huang, Matilda McDaniel, Katie Huang, Lan-Hsuan Lee, Su-Ju LinUSA). Both IP and no-Ab samples were incubated with 60 μL of ChIP-grade protein G beads (Cell Signaling 9007S) for 2 h at 4 °C and then washed as previously described. DNA was then eluted from the beads two times

(4) Statin prevents cancer development in chronic inflammation by blocking interleukin 33 expressionResearch SquareFebruary 27, 2023Jong Ho Demehri, Jong Ho Mortaja, Heehwa Mortaja, Marjan Azin, Marjan Wang, Michael Collier, Michael Mandinova, Yevgeniy Mandinova, Yevgeniy Mino-Kenudson, Shadmehr Mino-Kenudsonwith a 30 μL 50% slurry of ChIP-Grade Protein-G agarose beads (Cell Signaling Technology, catalog no. 9007S) for 3 hours. Samples were precipitated and washed three times with wash buffer containing 20 mM Tris/HCl

(5) LncRNA Gm26917 regulates inflammatory response in macrophages by enhancing Annexin A1 ubiquitination in LPS-induced acute liver injuryFront PharmacolNovember 1, 2022Qing Zhao, Meng-Fei Sheng, Yao-Yun Wang, Xing-Yu Wang, Wei-Yi Liu, Yuan-Yuan Zhang, Tiao-Ying Ke, Shu Chen, Gao-Zong Pang, Liang Yong, et al.Immunoprecipitation was performed using anti-IgG antibody (bsm-33179M-HRP), anti-FOXM1 antibody (ab207298) and ChIP-Grade Protein G Agarose Beads (Cell Signaling Technology). Associated DNA was then purified using a PCR clean-up kit (Axygen) and

(6) ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosisAutophagyNovember 1, 2022Zhang J, Wang H, Chen H, Li H, Xu P, Liu B, Zhang Q, Lv C, Song X.Antibody/antigen complexes were recovered with Protein G agarose beads (Cell signaling technology, 9007) for 2 h at 4°C. Two sequential elutions were performed. Eluted chromatin was diluted 1:3 with lysis

(7) ELK1-mediated YTHDF1 drives prostate cancer progression by facilitating the translation of Polo-like kinase 1 in an m6A dependent mannerInternational Journal of Biological SciencesOctober 18, 2022Peizhang Li, Yuanping Shi, Dajun Gao, Huan Xu, Yun Zou, Zhong Wang, Wenzhi Lifor 2 hours. The immunoprecipitated DNA was retrieved from ChIP-Grade Protein G Agarose Beads (CST, 9007). Statistics analysis All statistical analysis were performed using SPSS (22.0). The data are expressed

(8) MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling PathwayMolecules (Basel, Switzerland)August 22, 2022Xu P, Zhang H, Li H, Liu B, Li R, Zhang J, Song X, Lv C, Li H, Chen M.antibody/antigen complexes were recovered with protein G agarose beads (Cell signaling technology, 9007, Danvers, MA, USA) for 2 h at 4 °C. After two sequential elutions, 0.2 M NaCl was added to the eluent

(9) GCN5L1 impairs diastolic function in mice exposed to a high fat diet by restricting cardiac pyruvate oxidationPhysiological ReportsAugust 3, 2022Dharendra Thapa et al.Cell Signaling Technology. Immunocaptured proteins were isolated using Protein‐G agarose beads (Cell Signaling Technology, #9007), washed multiple times with CHAPS buffer, and then eluted in LDS sample buffer (Life Technologies)

(10) Paracrine signal emanating from stressed cardiomyocytes aggravates inflammatory microenvironment in diabetic cardiomyopathyiScienceMarch 18, 2022Kaur N, Ruiz-Velasco A, Raja R, Howell G, Miller JM, Abouleisa RRE, Ou Q, Mace K, Hille SS, Frey N, et al.investigate protein association, immunoprecipitations were performed with Protein G agarose beads (9007, Cell Signaling) following manufacturer’s instructions. Briefly, tissue was lysed with RIPA buffer (150

(11) Acinetobacter baumannii up-regulates LncRNA-GAS5 and promotes the degradation of STX17 by blocking the activation of YY1VirulenceDecember 1, 2021An Z, Ding W.Signaling, #46395) or control antibody (Cell Signaling, #2729) at 4°C with rotation and incubated with ChIP-grade protein G agarose beads for 2 h. The bound DNA was then analyzed by real-time PCR using primer sequences spanning the STX17

(12) Integrative genome-wide analysis of dopaminergic neuron-specific PARIS expression in Drosophila dissects recognition of multiple PPAR-γ associated gene regulationScientific reportsNovember 2, 2021Yazar V, Kang SU, Ha S, Dawson VL, Dawson TM.or histone antibody (Cell signaling) as a positive control. Antibody-DNA complexes were isolated by ChIP grade protein G-agarose beads, eluted from the beads, and digested by 40 μg of Proteinase K for 2 h at 65 °C, followed by spin column-based

(13) HIF2α promotes tumour growth in clear cell renal cell carcinoma by increasing the expression of NUDT1 to reduce oxidative stressClinical and translational medicineNovember 1, 2021Shi J, Xiong Z, Wang K, Yuan C, Huang Y, Xiao W, Meng X, Chen Z, Lv Q, Miao D, et al.2729, Boston, USA) was used to pretreat the cell lysate and Chip‐Grade protein G Agarose Beads (CST, 9007S, Boston, USA). Incubate an anti‐NUDT1 antibody (ABclonal, A13330, Wuhan, China) overnight at 4°C. Also

(14) CDK4 has the ability to regulate Aurora B and Cenpp expression in mouse keratinocytesOncology lettersOctober 1, 2021Lee SH, Rodriguez LRL, Majumdar R, De Marval PLM, Rodriguez-Puebla ML.(New England Biolabs) were added to DNA samples and incubated overnight at 4°C. Thirty microliters of ChIP-grade protein G agarose beads (Cell Signaling Technology, Inc.) were added to each sample and incubated for 2 h at 4°C, followed by

(15) ALKBH1-demethylated DNA N6-methyladenine modification triggers vascular calcification via osteogenic reprogramming in chronic kidney diseaseThe Journal of clinical investigationJuly 15, 2021Ouyang L, Su X, Li W, Tang L, Zhang M, Zhu Y, Xie C, Zhang P, Chen J, Huang H.4°C, followed by incubation with 50 μL ChIP-Grade Protein G Agarose beads (Cell Signaling Technology, 9007S) for 2 hours at 4°C with gentle rotation. The crosslinked DNA fragments were eluted, reversed, and purified.

(16) miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3Stem Cells InternationalJuly 15, 2021Changming Zhao et al.buffer containing protease inhibitors. The total cell extracts were mixed with precleared protein G (#9007, Cell Signaling Technology, USA) and then incubated 1 μg primary antibody against β-catenin or negative

(17) Bortezomib suppresses self-renewal and leukemogenesis of leukemia stem cell by NF-ĸB-dependent inhibition of CDK6 in MLL-rearranged myeloid leukemiaJournal of cellular and molecular medicineMarch 1, 2021Zhou B, Qin Y, Zhou J, Ruan J, Xiong F, Dong J, Huang X, Yu Z, Gao S.12 000 × g at 4°C to transfer the supernatants to new microcentrifuges. Protein A/G Agarose Beads (#9007, CST) was added to the supernatants to reduce the nonspecific background. Then, protein‐DNA complexes

(18) Cancer-associated exportin-6 upregulation inhibits the transcriptionally repressive and anticancer effects of nuclear profilin-1Cell reportsFebruary 16, 2021Zhu C, Kim SJ, Mooradian A, Wang F, Li Z, Holohan S, Collins PL, Wang K, Guo Z, Hoog J, et al.antibody (DSHB, #12E6) overnight at 4°C with rotation and mixed with 30 μL of the protein G beads (CST, #9007S) at 4°C for 1-2 hr. The beads were then washed 3-5 times with IP buffer (40 mM Tris-Cl, pH 7.5, 150

(19) The glucocorticoid receptor recruits the COMPASS complex to regulate inflammatory transcription at macrophage enhancersCell reportsFebruary 9, 2021Greulich F, Wierer M, Mechtidou A, Gonzalez-Garcia O, Uhlenhaut NH.antibody, or rabbit IgG, antibody-bait complexes were captured by ChIP-Grade Protein G Agarose Beads (#9007 Cell Signaling Technology), washed three times with wash buffer A (50mM HEPES pH 7.5, 140mM NaCl, 1%

(20) hCINAP serves a critical role in hypoxia‑induced cardiomyocyte apoptosis via modulating lactate production and mitochondrial‑mediated apoptosis signalingMolecular medicine reportsFebruary 1, 2021Xie H, Xu G, Gao Y, Yuan Z.(1 µg; cat. no. NB100-105; Novus Biologicals, LLC) and ChIP-Grade Protein G Agarose Beads (cat. no. 9007; Cell Signaling Technology, Inc.) incubated at 4°C for 2 h. Subsequently, chromatin complexes were eluted

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2/14/2023Protein-G Agarose Beads Works Well Liyong Z.Upstate Biotechnology’s Chromatin Immunoprecipitation (ChIP) Assay Kit now has the cat # 17-295 since it was merged by Sigma-Aldrich. Chromatin is comprised of nucleosome subunits, each of which ... Read Review